Device

Part:BBa_K1065105

Designed by: Gabriele Girelli   Group: iGEM13_UNITN-Trento   (2013-08-14)

lac_promoter + RBS + SAM synthetase

SAM synthetase enzyme catalyzes the reaction of synthesis of S-adenosyl-L-methionine (S-AdoMet, a.k.a. SAM). This reaction is part of the ethylene biosynthesis pathway I, which is present in plants [1].

ATP + L-Met + H2O -> S-AdoMet + Pi + PPi [2]

S-AdoMet is an intermediate in the ethylene biosynthesis pathway I (plants), a methyl donor for many cellular molecules (Methylated Acceptors) and in the methyl salicylate (MeSA) biosynthesis pathway, and the precursor of the polyamine synthesis pathway (Spermidine/Spermine biosynthesis pathway) [3].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None